Plasma antithrombin activity of MD 805 determined by chromogenic substrate during hemodialysis.

Biological Activity Analysis of Native and Recombinant Streptokinase Using Clot Lysis and Chromogenic Substrate Assay

Determination of streptokinase activity is usually accomplished through two assay methods: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...

Biological Activity Analysis of Native and Recombinant Streptokinase Using Clot Lysis and Chromogenic Substrate Assay

Determination of streptokinase activity is usually accomplished through two assay methods: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...

The antithrombin activity of human plasma.

Abrams, A., Kegeles, G. & Hottle, G. A. (1946). J. biol. Chem. 164, 63. Balls, A. K. & Lineweaver, H. (1939). J. biol. Chem. 130, 669. Bronfenbrenner, J. J. & Schlesinger, M. J. (1924). J. exp. Med. 39, 509. Buehler, H. J., Schantz, E. J. & Lamanna, C. (1947). J. biol. Chem. 169, 295. Chistyakov, F. M. & Rodopulo, A. R. (1943). J. Microbiol., Mo8cov, no. 9, 88 in Chem. Abstr. 39, 3323. Dowmont,...

Centrifugal analysis for plasma kallikrein activity, with use of the chromogenic substrate S-2302.

The amidolytic activity of activated kallikrein in plasma can be measured by use of the chromogenic substrate, S-2302 (H-D-Pro-Phe-Arg-pNA). Plasma prekallikrein was activated to kallikrein by exposure to 50 mg/L dextran sulfate in acetone/water (35/65 by vol) at 0 degrees C for 15 min. The acetone slows anti-kallikrein activity and increases the kallikrein activity by 30%. The 37 degrees C rea...

Changes of Plasma Angiotensin-Converting Enzyme Activity during Hemodialysis*